Table 1 Primers sequences used for Real-Time PCR
From: In vitro study on antioxidant and lipid-lowering activities of tobacco polysaccharides
Gene | Genbank Accession | Primer sequences (5' to 3') | Amplicon size (bp) |
|---|---|---|---|
GAPDH | NM002046.5 | CCATGACAACTTTGGTATCGTGGAA GGCCATCACGCCACAGTTTC | 107 |
PPAR-α | CR456547.1 | CCCTCCTCGGTGACTTATC | 111 |
GTAATGATAGCCTGAGGCCTTGT | |||
CYP7A1 | NM_000780.4 | CCTCCAGTCTCCTCTAACTCA | 126 |
GTCCCGCCTTGTAAGATCTCT | |||
CPT1A | NM_001876.4 | CACATTCAGGCAGCAAGAGC | 121 |
CGGAGCAGAGTGGAATCGT |