Table 3 Forward Primer for Real-Time PCR and Conditions
From: In vitro study on antioxidant and lipid-lowering activities of tobacco polysaccharides
Gene | Forward Primer and Universal Primer (5' to 3') | Annealing (℃) |
|---|---|---|
hsa-miR-7-3p-F | CGCGCAACAAATCACAGTCTG | 60 |
hsa-miR-17-3p-F | GCGACTGCAGTGAAGGCACTT | 60 |
hsa-miR-33a-5p-F | CGCGGTGCATTGTAGTTGC | 60 |
hsa-miR-30a-3p-F | GCGCTTTCAGTCGGATGTTTG | 60 |
hsa-miR-155-3p-F | CGCGCTCCTACATATTAGCA | 60 |
External Reference-F | CACCGGGTGTAAATCAGCTTG | 60 |
Universal Reverse Primer (micro-R) | AGTGCAGGGTCCGAGGTATT | 60 |