Table 4 Real-Time PCR conditions of miRNAs
From: In vitro study on antioxidant and lipid-lowering activities of tobacco polysaccharides
Conditions | Reaction systems (20 μL) |
|---|---|
Sterile Distilled Water (SDW) | 8.0 μL |
Power SYBR® Green Master Mix | 10.0 μL |
Forward Primer (10 μM) | 0.5 μL |
micro-R (10 μM) | 0.5 μL |
cDNA | 1.0 μL |