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Table 2 Mutants identified through evolutionary analysis

From: Enhancing the endo-activity of the thermophilic chitinase to yield chitooligosaccharides with high degrees of polymerization

No.

Site

AA (WT)

Max. AA (GH19)

Diff. score

1

264

M

L

0.0768

2

278

H

L

0.0511

3

44

Y

L

0.0452

4

74

H

L

0.0446

5

98

W

L

0.0385

6

170

Y

L

0.0372

7

49

Y

A

0.0355

8

56

Y

L

0.0352

9

79

F

A

0.0335

10

269

H

L

0.0335

11

402

I

L

0.0333

12

53

W

L

0.0327

13

442

I

L

0.0309

14

50

F

L

0.0300