Modeling the behavior of monoclonal antibodies on hydrophobic interaction chromatography resins

Bioresources and Bioprocessing

Table 1 Retention factor values for each mAb A-F across different HIC resins

	mAb A	mAb B	mAb C	mAb D	mAb F	mAb G
POROS Ethyl	0.16	0.13	0.16	0.17	0.14	0.29
POROS Benzyl	1.00	0.39	0.89	1.06	0.61	4.15
TOYOPearl PPG	0.14	1.00	0.44	0.15	0.11	0.17
TOYOPearl Butyl	0.79	0.39	0.73	0.66	0.41	4.78
TOYOPearl Phenyl	2.79	0.85	3.58	1.92	1.71	15.87
Phenyl FF LS	0.38	0.29	0.59	0.45	0.43	0.91
Butyl S FF	0.12	0.10	0.12	0.14	0.13	0.32
Capto Octyl	0.25	0.23	0.24	0.23	0.23	0.38
Capto Butyl	0.75	0.57	0.95	4.33	0.56	1.99
Capto Butyl ImPres	0.35	0.24	0.33	0.31	0.29	0.97
Capto Phenyl ImPres	3.48	1.84	6.15	3.56	3.21	13.81

Retention factors were measured using a 50 mM sodium citrate 350 mM ammonium sulfate pH 7 mobile phase