Fig. 7

Characterization of wild-type and variants of LmrR. A Effects of temperature on the catalytic activity. Reaction condition: 1 mL of water containing purified wild-type and variants (0.3 mg/mL), p-nitrobenzaldehyde (12 mM), nitromethane (48 mM) at 20 °C, 30 °C, 40 °C, 50 °C, 60 °C, and 70 °C; B Thermal stability of LmrR at different temperatures. The WT incubated in the water at 30 °C, 40 °C, 50 °C, 60 °C, respectively. Reaction conditions: 1 mL of water containing purified WT (0.3 mg/mL), p-nitrobenzaldehyde (12 mM), nitromethane (48 mM) at 40 °C; C Comparison of thermal stability of wild type and variants of LmrR. he WT and different variants were incubated in the water at 60 ℃ for 30 min, 60 min, 90 min, 120 min, 150 min, and 180 min, respectively. Reaction condition: 1 mL of water containing purified wild-type and variants (0.3 mg/mL), p-nitrobenzaldehyde (12 mM), nitromethane (48 mM) at 40 °C; D Effects of pH on the catalytic activity. Reaction condition: 1 mL of different buffer (pH 6.0, 7.0, 7.5, 8.0, 8.5, 9.0) containing purified wild-type and variants (0.3 mg/mL), p-nitrobenzaldehyde (12 mM), nitromethane (48 mM) at 40 °C