Rationally introducing non-canonical amino acids to enhance catalytic activity of LmrR for Henry reaction

Bioresources and Bioprocessing

Table 2 Alanine scanning

^aWhen the mutation energy is greater than 0.5, the Effect is destabilizing, indicating that the mutation will lead to a decrease in the affinity between the enzyme and substrates; when the mutation energy is between − 0.5 ~ + 0.5, the Effect is neutral, indicating that the mutation has no effect on the affinity between the enzyme and substrates