Bioresources and Bioprocessing

Table 3 Saturated mutation scanning

From: Rationally introducing non-canonical amino acids to enhance catalytic activity of LmrR for Henry reaction

Mutation	Mutation Energy^a (kcal/mol)	Effect
A11ʹK	− 1.09	Stabilizing
A11ʹF	− 0.92	Stabilizing
A11ʹH	− 0.82	Stabilizing
V15ʹW	− 0.82	Stabilizing
A11ʹM	− 0.81	Stabilizing
A11ʹQ	− 0.78	Stabilizing
A11ʹL	− 0.73	Stabilizing
V15W	− 0.72	Stabilizing
A11ʹE	− 0.65	Stabilizing
A92ʹQ	− 0.55	Stabilizing
A92ʹR	− 0.52	Stabilizing
A11ʹR	− 0.50	Neutral
A11ʹN	− 0.48	Neutral
V15Y	− 0.47	Neutral
V15F	− 0.45	Neutral
V15ʹF	− 0.45	Neutral
V15ʹY	− 0.44	Neutral
A92ʹE	− 0.43	Neutral
A92ʹW	− 0.42	Neutral
A11ʹW	− 0.41	Neutral

^aWhen the mutation energy is less than − 0.5, the Effect is stabilizing, indicating that the mutation will lead to an increase in the affinity between the enzyme and substrates; when the mutation energy is between − 0.5 ~ + 0.5, the Effect is neutral, indicating that the mutation has no effect on the affinity between the enzyme and substrates

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