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Bioresources and Bioprocessing

Table 4 Kinetic parameters of wild type and variants of LmrR for Henry reaction

From: Rationally introducing non-canonical amino acids to enhance catalytic activity of LmrR for Henry reaction

Mutation

Vmax (mM/min)

Km (mM)

kcat (min−1)

kcat/ Km (mM−1 min−1)

LmrR

1.29

9.30

58.05

6.24

V15F

1.96

8.28

88.20

10.65

V15CNF

2.41

10.08

108.45

10.76

V15pAF

2.15

8.86

96.75

10.92

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