Table 1 NA induced mutagenesis of B. licheniformis (wild-type IIB-isl9) and development of resistance against L-cysteine HCl for improved PHB production
From: Kinetics and synthesis of poly(3-hydroxybutyrate) by a putative-mutant of Bacillus licheniformis
Different Treatments | Death rate (%) | No. of survivor colonies | Strain coding | Range of PHB production (g/l) |
|---|---|---|---|---|
Wild-type IIB-isl9 | 0.56 ± 0.12 | |||
NA conc. (mM)$ | ||||
50 | 70 | 6 | NA-1, NA-2, NA-3, NA-4, NA-5, NA-6 | 0.03–0.38 |
100 | 75 | 2 | NA-7, NA-8 | 0.31–0.45 |
150 | 80 | 5 | NA-9, NA-10, NA-11, NA-12, NA-13 | 0.46–0.64 |
200 | 85 | 4 | NA-14, NA-15, NA-16, NA-17 | 0.65–0.81 |
250 | 85 | 3 | NA-18, NA-19, NA-20 | 0.82–1.09 |
300 | 90 | 1 | NA-21 | 1.12 ± 0.3 |
NA exposure time (min)˄ | ||||
5 | 80 | - | - | - |
10 | 85 | 4 | NA-t1, NA-t2, NA-t3, NA-t4 | 0.97–1.68 |
15 | 90 | 3 | NA-t5, NA-t6, NA-t7 | 1.08–2.95 |
20 | 90 | - | - | - |
25 | 95 | 1 | NA-t8 | 3.22 ± 0.51 |
30 | 100 | - | - | - |
L-Cysteine HCl conc. (ppm) | ||||
2 | 85 | - | - | - |
4 | 98 | 1 | NA-cys4 | 3.19 ± 0.43 |
6 | 100 | - | - | - |
8 | 100 | - | - | - |
10 | 100 | - | - | - |
12 | 100 | - | - | - |