From: Kinetics and synthesis of poly(3-hydroxybutyrate) by a putative-mutant of Bacillus licheniformis
Kinetic parameters | Different incubation periods (h) | |||||||
---|---|---|---|---|---|---|---|---|
24 | 48 | 72 | 96 | |||||
IIB-isl9 | NA-cys4 | IIB-isl9 | NA-cys4 | IIB-isl9 | NA-cys4 | IIB-isl9 | NA-cys4 | |
Yield coefficients | ||||||||
Growth yield coefficients | ||||||||
Yx/s (g biomass/g) | 2.062 ± 0.10 | 1.231 ± 0.06 | 1.583 ± 0.08 | 1.132 ± 0.06 | 0.897 ± 0.04 | 1.031 ± 0.05 | 0.511 ± 0.02 | 0.709 ± 0.04 |
Ys/x (g/g biomass) | 0.485 ± 0.02 | 0.813 ± 0.04 | 0.632 ± 0.03 | 0.883 ± 0.04 | 1.115 ± 0.06 | 0.972 ± 0.05 | 1.961 ± 0.10 | 1.411 ± 0.07 |
Product yield coefficients | ||||||||
Yp/x (g/g biomass) | 0.428 ± 0.02 | 1.091 ± 0.05 | 0.531 ± 0.03 | 1.254 ± 0.06 | 0.532 ± 0.03 | 1.238 ± 0.06 | 0.393 ± 0.02 | 1.411 ± 0.07 |
Yp/s (g/g) | 0.882 ± 0.04 | 1.341 ± 0.07 | 0.841 ± 0.04 | 1.421 ± 0.07 | 0.477 ± 0.02 | 1.274 ± 0.06 | 0.201 ± 0.01 | 1.001 ± 0.05 |
Specific rate constants | ||||||||
Specific rate constants for product formation | ||||||||
qp*1 (g/g/h) | 0.064 ± 0.03 | 0.066 ± 0.03 | 0.044 ± 0.02 | 0.191 ± 0.01 | 0.011 ± 0.01 | 0.093 ± 0.05 | 0.002 ± 0.01 | 0.039 ± 0.01 |
qp*2 (g/g/h) | 0.031 ± 0.02 | 0.212 ± 0.01 | 0.028 ± 0.01 | 0.168 ± 0.01 | 0.012 ± 0.01 | 0.091 ± 0.04 | 0.003 ± 0.01 | 0.055 ± 0.03 |
Specific rate constants for biomass development and glucose consumption | ||||||||
qx (g/g/h) | 0.148 ± 0.01 | 0.239 ± 0.01 | 0.082 ± 0.04 | 0.152 ± 0.01 | 0.021 ± 0.01 | 0.075 ± 0.03 | 0.004 ± 0.01 | 0.028 ± 0.01 |
qs (g/g/h) | 0.035 ± 0.01 | 0.158 ± 0.01 | 0.033 ± 0.02 | 0.118 ± 0.01 | 0.026 ± 0.01 | 0.071 ± 0.04 | 0.016 ± 0.01 | 0.055 ± 0.03 |
Volumetric rates | ||||||||
Volumetric rates for product formation | ||||||||
Qp(g/l/h) | 0.072 ± 0.01 | 0.194 ± 0.01 | 0.052 ± 0.03 | 0.134 ± 0.01 | 0.023 ± 0.01 | 0.073 ± 0.04 | 0.008 ± 0.01 | 0.039 ± 0.01 |
Volumetric rates for biomass development and glucose consumption | ||||||||
Qx (g biomass/l/h) | 0.168 ± 0.01 | 0.178 ± 0.01 | 0.097 ± 0.05 | 0.107 ± 0.01 | 0.043 ± 0.02 | 0.059 ± 0.03 | 0.021 ± 0.01 | 0.028 ± 0.01 |
Qs (g/l/h) | 0.081 ± 0.04 | 0.145 ± 0.01 | 0.061 ± 0.03 | 0.095 ± 0.05 | 0.048 ± 0.02 | 0.057 ± 0.03 | 0.041 ± 0.02 | 0.039 ± 0.02 |