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Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Identification, heterologous expression and characterization of a new unspecific peroxygenase from Marasmius fiardii PR-910

Fig. 2

A Strategy of multi-copy strains construction. B NBD activities of multi-copy strains on shaking flask scale. C The high-cell-density fermentation of P. pastoris MFI2c in a 5 L bioreactor. The red arrow showed that 0.5% (m/v) yeast extract and 1.0% (m/v) tryptone were collaboratively supplemented at that time. D SDS-PAGE analysis of interval sampling.1–9 were the samples of 30 h, 60 h, 84 h, 108 h, 146 h, 180 h, 200 h, 210 h and 220 h. E Supernatant after centrifugation. F Assays of purified and N-deglycosylation rMfiUPO. 1, Purified rMfiUPO; 2, N-deglycosylation rMfiUPO and Endo H; 3, Endo H

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