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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Identification, heterologous expression and characterization of a new unspecific peroxygenase from Marasmius fiardii PR-910

Fig. 3

A pH activity profiles of rMfiUPO. Reactions were analyzed in citric acid/dibasic sodium phosphate buffer (pH 2.2 ~ 5.0) and potassium phosphate (pH 5.5 ~ 10.0) buffers. B Thermostability of rMfiUPO towards ABTS and NBD. Each point represents the mean and standard deviation of 3 independent experiments. C The relative activities of rMfiUPO in organic cosolvents were assessed with 2 mM H2O2 and 0.3 mM ABTS in 100 mM sodium phosphate/citrate buffer (pH 4.0) containing the corresponding concentration of cosolvent. D The stabilities of rMfiUPO after incubation for 40 h in 50% organic cosolvents were assessed by incubating enzyme samples in 100 mM potassium phosphate buffer (pH 7.0) containing 50% (vol/vol) organic cosolvent in screw-cap vials. After 40 h, aliquots were removed and analyzed in an activity assay with 2 mM H2O2 and 0.3 mM ABTS in 100 mM sodium phosphate/citrate buffer (pH 4.0)

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