Fig. 6From: Efficient expression of an alkaline pectin lyase from Bacillus licheniformis in Pichia pastorisEffects of gene dosage on PMGL-Ba expression. (A) Schematic of different gene doses. Red is the PMGL-Ba gene, light green and purple are screening markers, and â‘ â‘¡ is the gene integration order. (B) Growth curves of strains corresponding to different gene doses. (C) Effect of different gene doses on total protein concentration of PMGL-Ba and SDS-PAGE analysis. (D) Effect of different gene doses on PMGL-Ba enzyme activityBack to article page