Skip to main content
Bioresources and Bioprocessing
Fig. 2 | Bioresources and Bioprocessing

Fig. 2

From: Reconstruction the feedback regulation of amino acid metabolism to develop a non-auxotrophic l-threonine producing Corynebacterium glutamicum

Fig. 2

Selection of the strong promoters for overexpressing lysC-asd and hom-thrB operons. a A tailored reporter system for screening promoters for overexpressing lysC-asd and hom-thrB operons. The reporter gene was assembled by fusing the first 180 bp of target gene (hom or lysC) with a flexible linker (GGGGS)3 and a rfp gene. b Strength analysis of different promoters using the tailored reporter system. Data are presented as mean values +/− SD (n = 3 independent experiments). ***P < 0.001, student’s two-tailed t-test

Back to article page