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Bioresources and Bioprocessing
Fig. 6 | Bioresources and Bioprocessing

Fig. 6

From: Reconstruction the feedback regulation of amino acid metabolism to develop a non-auxotrophic l-threonine producing Corynebacterium glutamicum

Fig. 6

Overexpression of l-threonine exporter and homoserine kinase to enhance l-threonine production and reduce by-product accumulation. The 24-deep-well plate fermentation was performed at 30 °C for 36 h with 0.5 mM IPTG. Control, ZcglT5 derivative harboring pEC-XK99E empty plasmid; ZcglT6, ZcglT5 derivative harboring pEC-thrE plasmid; ZcglT7, ZcglT5 derivative harboring pEC-serE plasmid; ZcglT8, ZcglT5 derivative harboring pEC-rhtC plasmid; ZcglT9, ZcglT5 derivative harboring pEC-rhtCthrB plasmid. Thr, l-threonine; Lys, l-lysine; Ile, l-isoleucine; Gly, glycine; Hse, l-homoserine. Data are presented as mean values +/− SD (n = 3 independent experiments). ***P < 0.001, student’s two-tailed t-test

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