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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: A straightforward chemobiocatalytic route for one-pot valorization of glucose into 2,5-bis(hydroxymethyl)furan

Fig. 3

Comparison of BHMF synthesis before (solid symbols) and after (empty symbols) codon optimization of MgADH1 (A), SDS-PAGE analysis (B) and specific activity of the supernatant (C) before and after codon optimization. Reaction conditions (A): 200 mM HMF, 1 equiv. glucose, 60 mg/mL cells, 4 mL Tris-HCl buffer (0.1 M, pH 8), 30 °C, 200 rpm; (B) lane M: protein marker; before optimization: lane 1 (all proteins), lane 2 (supernatant), lane 3 (precipitant); after optimization: lane 4 (all proteins), lane 5 (supernatant), lane 6 (precipitant); (C) 5 mM HMF, 0.15 mM NADH, appropriate supernatant, Tris-HCl buffer (50 mM, pH 8), 35 °C

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