Fig. 4From: A straightforward chemobiocatalytic route for one-pot valorization of glucose into 2,5-bis(hydroxymethyl)furanBHMF synthesis at high substrate concentrations (A) and fed-batch synthesis of BHMF (B). Reaction conditions (A): 200–500 mM HMF, 1 equiv. glucose, 60 mg/mL cells, 4 mL Tris-HCl buffer (0.1 M, pH 8), 30 °C, 200 rpm; (B) 8-mL scale, arrows refer to supplementation of approximately 200 mM fresh HMF and 1 equiv. glucoseBack to article page