Influences of 3-hydroxypropionaldehyde and lactate on the production of 1,3-propanediol by Klebsiella pneumoniae

Bioresources and Bioprocessing

Table 1 Results of 1,3-propanediol fed-batch fermentation by KG1 and KG1Δldh with different initial concentrations of glycerol

Strain and initial glycerol concentration (g/L)		Lactate	3-HPA (12 h)	Biomass	2,3-BD	1,3-PD	1,3-PD yield
Strain and initial glycerol concentration (g/L)		(mM)	(mM)	(g/L)	(mM)	(mM)	(%)
KG1	20	18.9 ± 1.1	<1.5	6.9 ± 0.6	242 ± 13	872 ± 43	64 ± 2
KG1∆ldh	20	4.9 ± 0.5	<2.0	7.1 ± 0.7	261 ± 9	884 ± 38	65 ± 2
KG1	30	31.4 ± 2.5	10.9 ± 1.0	6.5 ± 0.3	213 ± 17	808 ± 48	59 ± 2
KG1∆ldh	30	55.3 ± 5.6 (+50)	11.9 ± 1.3	6.6 ± 0.3	240 ± 20	804 ± 48	59 ± 2
KG1	40	51.3 ± 4.0	14.6 ± 1.2	5.9 ± 0.4	190 ± 12	740 ± 32	55 ± 3
KG1	40	101.3 ± 9.0 (+50)	14.5 ± 1.2	5.3 ± 0.4	195 ± 11	742 ± 45	52 ± 3
KG1∆ldh	40	4.8 ± 0.7	15.4 ± 1.3	5.7 ± 0.3	222 ± 19	748 ± 30	56 ± 2
KG1∆ldh	40	54.8 ± 4.2 (+50)	13.5 ± 1.0	5.9 ± 0.4	225 ± 15	747 ± 54	55 ± 3

(+50) = 50 mM lactate adding into the fermentation media in the late stage of fermentation. Yield of 1,3-propanediol is on basis of mass. The date are final values except 3-HPA; the data of 3-HPA are peak values in 12 h of fermentation. Values are the mean values and standard deviations of three parallel experiments.