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Table 1 Results of 1,3-propanediol fed-batch fermentation by KG1 and KG1Δldh with different initial concentrations of glycerol

From: Influences of 3-hydroxypropionaldehyde and lactate on the production of 1,3-propanediol by Klebsiella pneumoniae

Strain and initial glycerol concentration (g/L) Lactate 3-HPA (12 h) Biomass 2,3-BD 1,3-PD 1,3-PD yield
(mM) (mM) (g/L) (mM) (mM) (%)
KG1 20 18.9 ± 1.1 <1.5 6.9 ± 0.6 242 ± 13 872 ± 43 64 ± 2
KG1∆ldh 20 4.9 ± 0.5 <2.0 7.1 ± 0.7 261 ± 9 884 ± 38 65 ± 2
KG1 30 31.4 ± 2.5 10.9 ± 1.0 6.5 ± 0.3 213 ± 17 808 ± 48 59 ± 2
KG1∆ldh 30 55.3 ± 5.6 (+50) 11.9 ± 1.3 6.6 ± 0.3 240 ± 20 804 ± 48 59 ± 2
KG1 40 51.3 ± 4.0 14.6 ± 1.2 5.9 ± 0.4 190 ± 12 740 ± 32 55 ± 3
KG1 40 101.3 ± 9.0 (+50) 14.5 ± 1.2 5.3 ± 0.4 195 ± 11 742 ± 45 52 ± 3
KG1∆ldh 40 4.8 ± 0.7 15.4 ± 1.3 5.7 ± 0.3 222 ± 19 748 ± 30 56 ± 2
KG1∆ldh 40 54.8 ± 4.2 (+50) 13.5 ± 1.0 5.9 ± 0.4 225 ± 15 747 ± 54 55 ± 3
  1. (+50) = 50 mM lactate adding into the fermentation media in the late stage of fermentation. Yield of 1,3-propanediol is on basis of mass. The date are final values except 3-HPA; the data of 3-HPA are peak values in 12 h of fermentation. Values are the mean values and standard deviations of three parallel experiments.