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Table 2 Experimental results from the central composite design

From: Recombinant d-galactose dehydrogenase partitioning in aqueous two-phase systems: effect of pH and concentration of PEG and ammonium sulfate

Assay

Factor level

Responses

X 1

X 2

pH (X3)

R(%)

Y(%)

PF

SA

1

1

−1

−1

220

72

47

185.317

2

1

−1

1

230

70.58

48.3

307.811

3

0

0

0

256.25

96.09

61

359.216

4

1

1

−1

195

62.83

43

121.153

5

0

−1.68

0

200

60

42.61

268.246

6

0

0

−1.68

195

60.43

53

278.261

7

−1

1

+1

163

59.62

30

296.022

8

0

0

0

268.75

92.80

58.9

373.913

9

−1

1

−1

181.25

42.64

35

129.486

10

1.68

0

0

259

62.83

39.81

155.593

11

−1.68

0

0

188

54.54

38

92.5069

12

1

1

+1

209

56.04

35.02

135.932

13

0

0

0

270

92.30

55

283.94

14

−1

−1

+1

205

65.75

36

103.347

15

0

1.68

0

175

62.68

26.25

89.3498

16

0

0

0

262.5

92.64

56.64

324.638

17

0

0

1.68

198

65.03

42.73

82.8794

18

0

0

0

270

96.35

61.76

332.703

19

−1

−1

−1

220

57.28

28.98

74.5062

20

0

0

0

268

79.41

63

333.025