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Table 2 Experimental results from the central composite design

From: Recombinant d-galactose dehydrogenase partitioning in aqueous two-phase systems: effect of pH and concentration of PEG and ammonium sulfate

Assay Factor level Responses
X 1 X 2 pH (X3) R(%) Y(%) PF SA
1 1 −1 −1 220 72 47 185.317
2 1 −1 1 230 70.58 48.3 307.811
3 0 0 0 256.25 96.09 61 359.216
4 1 1 −1 195 62.83 43 121.153
5 0 −1.68 0 200 60 42.61 268.246
6 0 0 −1.68 195 60.43 53 278.261
7 −1 1 +1 163 59.62 30 296.022
8 0 0 0 268.75 92.80 58.9 373.913
9 −1 1 −1 181.25 42.64 35 129.486
10 1.68 0 0 259 62.83 39.81 155.593
11 −1.68 0 0 188 54.54 38 92.5069
12 1 1 +1 209 56.04 35.02 135.932
13 0 0 0 270 92.30 55 283.94
14 −1 −1 +1 205 65.75 36 103.347
15 0 1.68 0 175 62.68 26.25 89.3498
16 0 0 0 262.5 92.64 56.64 324.638
17 0 0 1.68 198 65.03 42.73 82.8794
18 0 0 0 270 96.35 61.76 332.703
19 −1 −1 −1 220 57.28 28.98 74.5062
20 0 0 0 268 79.41 63 333.025