Table 2 Purification table of protease from Bacillus brevis MWB-01
Purification steps | Volume (ml) | Activity (U/ml) | Total activity (U) | Protein concentration (mg/ml) | Total protein (mg) | Specific activity (U/ml) | Fold | Yield (%) |
|---|---|---|---|---|---|---|---|---|
Crude enzyme preparation | 800 | 202 | 161,600 | 8.15 | 6412 | 25.2 | 1.0 | 100 |
Ammonium sulphate precipitation dialysed | 30 | 310 | 9,300 | 7.63 | 228.9 | 40.6 | 1.6 | 5.8 |
Gel filtration chromatography on Sephadex G-200 | 50 | 150 | 7,500 | 2.77 | 138.5 | 54.2 | 2.1 | 4.6 |