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Table 2 Purification table of protease from Bacillus brevis MWB-01

From: Purification and partial characterization of serine alkaline metalloprotease from Bacillus brevis MWB-01

Purification steps Volume (ml) Activity (U/ml) Total activity (U) Protein concentration (mg/ml) Total protein (mg) Specific activity (U/ml) Fold Yield (%)
Crude enzyme preparation 800 202 161,600 8.15 6412 25.2 1.0 100
Ammonium sulphate precipitation dialysed 30 310 9,300 7.63 228.9 40.6 1.6 5.8
Gel filtration chromatography on Sephadex G-200 50 150 7,500 2.77 138.5 54.2 2.1 4.6