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Table 1 The plasmids constructed in this study

From: Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. coli

Plasmids Parent plasmids Copies Promoter Antibiotic marker Expression strength
p40Trc-ls-gpps pTrcHis2B 40 Trc ampicillin 40
p15T7-ls-gpps pACYCDuet-1 15 T7 chloramphenicol 75
p40T7-ls-gpps pET28a+ 40 T7 kanamycin 200
p40Trc-gpps-ls pTrcHis2B 40 Trc ampicillin 40
p15T7-gpps-ls pACYCDuet-1 15 T7 chloramphenicol 75
p40T7-gpps-ls pET28a+ 40 T7 kanamycin 200
p40Trc-ls-Ecgpps pTrcHis2B 40 Trc ampicillin 40
p15T7-ls-Ecgpps pACYCDuet-1 15 T7 chloramphenicol 75
p40T7-ls-Ecgpps pET28a+ 40 T7 kanamycin 200
p40Trc-Ecgpps-ls pTrcHis2B 40 Trc ampicillin 40
p15T7-Ecgpps-ls pACYCDuet-1 15 T7 chloramphenicol 75
p40T7-Ecgpps-ls pET28a+ 40 T7 kanamycin 200
p40T7-dxs-idi pET21a+ 40 T7 ampicillin 200
p40T5-dxs-idi pQE30 40 T5 ampicillin 80
p40Trc-dxs-idi pTrcHis2B 40 Trc ampicillin 40
  1. The expression strengths of vectors were estimated using published values of promoter strength and copy number. Promoter strengths were calculated as Trc = 1, T5 = 2, T7 = 5 (Brosius et al. [21], Brunner et al. [22]). Gene copy number was assigned by published copy numbers for origin of replication for the different plasmids used.