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Table 2 List of engineered E. coli strains and the corresponding plasmids

From: Enhanced limonene production by optimizing the expression of limonene biosynthesis and MEP pathway genes in E. coli

Strain MEP pathwaydxs-idioperon Limonene pathwayls-gpps,gpps-ls,ls-Ecgpps, orEcgpps-lsoperons
Plasmid Expression strength Plasmid Expression strength
1 - 1 p40Trc-ls-gpps 40
2 - 1 p15T7-ls-gpps 75
3 - 1 p40T7-ls-gpps 200
4 - 1 p40Trc-gpps-ls 40
5 - 1 p15T7-gpps-ls 75
6 - 1 p40T7-gpps-ls 200
7 - 1 p40Trc-ls-Ecgpps 40
8 - 1 p15T7-ls-Ecgpps 75
9 - 1 p40T7-ls-Ecgpps 200
10 - 1 p40Trc-Ecgpps-ls 40
11 - 1 p15T7-Ecgpps-ls 75
12 - 1 p40T7-Ecgpps-ls 200
13 p40T7-dxs-idi 200 p15T7-ls-gpps 75
14 p40T5-dxs-idi 80 p15T7-ls-gpps 75
15 p40Trc-dxs-idi 40 p15T7-ls-gpps 75
  1. For a full plasmids description, see TableĀ 1.