Table 2 List of engineered E. coli strains and the corresponding plasmids
Strain | MEP pathwaydxs-idioperon | Limonene pathwayls-gpps,gpps-ls,ls-Ecgpps, orEcgpps-lsoperons | ||
|---|---|---|---|---|
Plasmid | Expression strength | Plasmid | Expression strength | |
1 | - | 1 | p40Trc-ls-gpps | 40 |
2 | - | 1 | p15T7-ls-gpps | 75 |
3 | - | 1 | p40T7-ls-gpps | 200 |
4 | - | 1 | p40Trc-gpps-ls | 40 |
5 | - | 1 | p15T7-gpps-ls | 75 |
6 | - | 1 | p40T7-gpps-ls | 200 |
7 | - | 1 | p40Trc-ls-Ecgpps | 40 |
8 | - | 1 | p15T7-ls-Ecgpps | 75 |
9 | - | 1 | p40T7-ls-Ecgpps | 200 |
10 | - | 1 | p40Trc-Ecgpps-ls | 40 |
11 | - | 1 | p15T7-Ecgpps-ls | 75 |
12 | - | 1 | p40T7-Ecgpps-ls | 200 |
13 | p40T7-dxs-idi | 200 | p15T7-ls-gpps | 75 |
14 | p40T5-dxs-idi | 80 | p15T7-ls-gpps | 75 |
15 | p40Trc-dxs-idi | 40 | p15T7-ls-gpps | 75 |