Skip to main content

Table 3 Achieved results in the transesterification reaction with biocatalyst

From: Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase

Run number

NaOH amount

Temperature

EtOH/oil

Viscosity

Selectivity

Conversion

TOF

Enzyme activity

1

25

30

3.5/12 (6:1)

10.5

34.9

47.7

79.5

0.24

2

25

30

3.5/12 (6:1)

13.7

23.6

32.9

54.83

0.35

3

25

30

3.5/12 (6:1)

12.8

24.1

31.8

53.00

0.36

4

25

30

3.5/12 (6:1)

13.3

25.6

33.9

56.50

0.33

5

25

30

3.5/12 (6:1)

12.8

29.1

35.6

59.33

0.32

6

12.5

35

3.5/12 (6:1)

13.8

14.5

20.1

33.50

0.57

7

12.5

30

3.5/12 (6:1)

12.3

45.4

57.9

96.50

0.20

8

12.5

25

3.5/12 (6:1)

14.6

21.4

25.8

43.00

0.44

9

12.5

40

3.5/12 (6:1)

20.9

7.6

4.0

6.67

2.85

10

12.5

30

1.75/12 (3:1)

18,8

17,9

27,4

45.67

0.42

11

12.5

20

3.5/12 (6:1)

18.1

17.5

22.4

37.33

0.51

12

12.5

30

2.3/12 (4:1)

20.0

17.6

21.4

35.67

0.53

13

12.5

30

2.9/12 (5:1)

10.6

34.1

47.7

79.50

0.24

14

12.5

30

3.5/12 (6:1)

10.4

39.1

51.9

86.50

0.22

15

12.5

30

4.1/12 (7:1)

17.9

14.1

16.8

28.00

0.68

16

12.5

30

4.7/12 (8:1)

16.3

17.1

21.8

36.33

0.52

  1. Viscosity (cSt), conversion (%), selectivity (%), turnover frequencies (TOF in μmol min−1 g cat−1), and enzyme activities (U/mg lipase) of the obtained biodiesel by using the same biocatalyst (0.5 g of N435) in successive reactions under the evaluated different experimental conditions: temperature (°C), NaOH amount (μl of NaOH 10 N solution), and EtOH/oil ratio (ml/ml or mol/mol).