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Table 3 Achieved results in the transesterification reaction with biocatalyst

From: Production of a biodiesel-like biofuel without glycerol generation, by using Novozym 435, an immobilized Candida antarctica lipase

Run number NaOH amount Temperature EtOH/oil Viscosity Selectivity Conversion TOF Enzyme activity
1 25 30 3.5/12 (6:1) 10.5 34.9 47.7 79.5 0.24
2 25 30 3.5/12 (6:1) 13.7 23.6 32.9 54.83 0.35
3 25 30 3.5/12 (6:1) 12.8 24.1 31.8 53.00 0.36
4 25 30 3.5/12 (6:1) 13.3 25.6 33.9 56.50 0.33
5 25 30 3.5/12 (6:1) 12.8 29.1 35.6 59.33 0.32
6 12.5 35 3.5/12 (6:1) 13.8 14.5 20.1 33.50 0.57
7 12.5 30 3.5/12 (6:1) 12.3 45.4 57.9 96.50 0.20
8 12.5 25 3.5/12 (6:1) 14.6 21.4 25.8 43.00 0.44
9 12.5 40 3.5/12 (6:1) 20.9 7.6 4.0 6.67 2.85
10 12.5 30 1.75/12 (3:1) 18,8 17,9 27,4 45.67 0.42
11 12.5 20 3.5/12 (6:1) 18.1 17.5 22.4 37.33 0.51
12 12.5 30 2.3/12 (4:1) 20.0 17.6 21.4 35.67 0.53
13 12.5 30 2.9/12 (5:1) 10.6 34.1 47.7 79.50 0.24
14 12.5 30 3.5/12 (6:1) 10.4 39.1 51.9 86.50 0.22
15 12.5 30 4.1/12 (7:1) 17.9 14.1 16.8 28.00 0.68
16 12.5 30 4.7/12 (8:1) 16.3 17.1 21.8 36.33 0.52
  1. Viscosity (cSt), conversion (%), selectivity (%), turnover frequencies (TOF in μmol min−1 g cat−1), and enzyme activities (U/mg lipase) of the obtained biodiesel by using the same biocatalyst (0.5 g of N435) in successive reactions under the evaluated different experimental conditions: temperature (°C), NaOH amount (μl of NaOH 10 N solution), and EtOH/oil ratio (ml/ml or mol/mol).