Figure 3

Repeated use of the immobilized BsE. The reaction was performed in a 50-ml three-necked flask containing 5.94 g dl-menthyl acetate, 25 mg SDS and 8 ml KPB (pH 8.0, 200 mM) and 2 ml n-pentanol, at 30°C and 300 rpm with mechanical stirring. After 24-h reaction, the reaction was terminated by filtration, and the recovered immobilized BsE was reused in next batch of reaction. Symbols: black square, relative activity (%); gray square, conversion after 24-h reaction.