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Table 1 Enantioselective hydrolysis of dl -menthyl acetate by immobilized BsE in different organic-aqueous biphasic systems

From: Efficient production of l-menthol in a two-phase system with SDS using an immobilized Bacillus subtilis esterase

Organic solvent

LogP

Conversion (%)

eep(%)

Evalue

Control

 

41

94

63

Methyl t-butyl ether

1.3

39

81

17

n-Pentanol

1.5

44

85

26

Pentyl acetate

2.1

15

89

20

Toluene

2.5

44

78

15

Carbon tetrachloride

2.8

37

70

10

n-Nonanol

3.6

50

63

8

n-Octane

4.4

32

61

5

n-Dodecane

6.1

32

53

4

  1. The biphasic reactions were performed by adding 1.98 g (10 mmol) of dl-menthyl acetate and 0.55 g immobilized BsE into a mixture of 8 ml KPB (pH 8.0, 200 mM) and 2 ml organic solvent at 30°C and 300 rpm for 48 h. The control reaction was performed in 10 ml KPB containing 10% (v/v) ethanol.