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Table 4 Enantioselective hydrolysis of dl -menthyl acetate at different substrate loads by immobilized BsE under the optimal reaction conditions

From: Efficient production of l-menthol in a two-phase system with SDS using an immobilized Bacillus subtilis esterase

Substrate loading

Reaction time (h)

eep(%)

Conversion (%)

Evalue

1.0 M/198 g l−1

48

97

43

158

2.0 M/396 g l−1

54

97

43

158

3.0 M/594 g l−1

72

97

40

120

  1. The reactions were performed at 30°C and 300 rpm for 48 h by adding different concentrations of dl-menthyl acetate, 0.55 g immobilized BsE, and 25 mg of SDS into a mixture of 8 ml KPB (pH 8.0, 200 mM) and 2 ml n-pentanol.