Table 1 Primer sequences used in the real-time fluorescence quantitative PCR
Gene | Prime sequences | Fragment size (bp) |
|---|---|---|
16S RNA | F:5′CTGGACTGTAACTGACGCTGA3′ | 80 |
R:5′CGTTTACGGCGTGGACTAC3′ | ||
ctfAB | F:5′CAGAAAACGGAATAGTTGGAATG3′ | 151 |
R:5′TGACCACCACGGATTAGTGAA3′ | ||
adhE | F:5′GTTTTGGCTATGTATGAGGCTGA3′ | 241 |
R:5′CAAGCGTGAAAGAAGGTGGTAT3′ | ||
bdhB | F:5′ACGCTTCTGCCATTCTATCC3′ | 175 |
R:5′ATTGCGGCACATCCAGATA3′ | ||
ask | F:5′GTATGGGATTTACTCCTCTTGG3′ | 63 |
R:5′CTGGGTCCATATCTCCACTTC3′ | ||
buk | F:5′TCCGCCTTTGCCGTTTA3′ | 194 |
R:5′ACATGGGTGGAGGTACTTCAGT3′ |