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Figure 1 | Bioresources and Bioprocessing

Figure 1

From: Characterization of abnZ2 (yxiA1) and abnZ3 (yxiA3) in Paenibacillus polymyxa, encoding two novel endo-1,5-α-l-arabinanases

Figure 1

Expression and purification of recombinant protein. The proteins were separated by 12.5% SDS-PAGE gels. (a) Analysis of the protein YxiA1 expression. Lane 1, crude cell lysate after cold osmotic shock of wild BL21; lane 2, crude cell lysate; lane 3, soluble fraction of the crude cell lysate; and lane 4, insoluble fraction of the crude cell lysate. (b) Analysis of the protein YxiA3 expression. Lane 1, crude cell lysate after cold osmotic shock of wild BL21; lane 2, crude cell lysate; lane 3, soluble fraction of the crude cell lysate; and lane 4, insoluble fraction of the crude cell lysate. (c) Recombinant YxiA1 purified by Ni-Sepharose column eluting with 50, 100, and 150 mM imidazole. (d) Recombinant YxiA3 purified by Ni-Sepharose column eluting with 50, 100, and 150 mM imidazole.

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