Table 1 Primers used in this work
Primer | Oligonucleotide sequence | Tm (°C) |
|---|---|---|
Abn-w6f | 5′-GGTGGATCC AAGCAATTATTTGTTCGTAA-3′ | 60.00 |
Abn-w6r | 5′-TATCTCGAG CAAATCCGCCAGTGC-3′ | 61.83 |
Abn-w7f | 5′-GGTGGATCC GTTATGCTGCTAATGCTG-3′ | 63.00 |
Abn-w7r | 5′-TTTCTCGAG ATCGCTGATACGTTTTCCC-3′ | 62.20 |