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Bioresources and Bioprocessing

Table 1 Effect of NAD + and FBP on the activity of LDH Tt

From: In vitro conversion of glycerol to lactate with thermophilic enzymes

FBP (mM)

NAD+(mM)

0

0.2

0.5

1

0

192 ± 4.2

2.51 ± 0.10

1.08 ± 0.03

0.31 ± 0.02

0.5

1,150 ± 15

980 ± 3.5

396 ± 6.2

230 ± 2.9

  1. Enzyme assays were performed with and without 0.5 mM FBP in the reaction mixture containing indicated concentrations of NAD+. Results were expressed as specific enzyme activities, which were assessed using a heat-treated cell-free extract and normalized by the protein concentration of the corresponding non-heated cell-free extract (U mg−1 protein).
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