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Bioresources and Bioprocessing
Figure 1 | Bioresources and Bioprocessing

Figure 1

From: Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies

Figure 1

SDS-PAGE and western blot analysis of mda-7/IL-24. (A) SDS-PAGE analysis of mda-7/IL-24 expressed in E. coli. Lane M, protein molecular weight markers; lanes 1 to 2, whole cell lysate of pET28a-mda-7/IL-24 uninduced and induced; lane 3, insoluble fractions of cell lysate after induction by IPTG; lane 4, soluble fractions of cell lysate after induction by IPTG. (B) Western blot analysis of mda-7/IL-24 protein. Lane 1, western blot analysis of mda-7/IL-24; lane 2, blank control without anti-human mda-7/IL-24 antibody.

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