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Table 3 Box-Behnken design of variables for process optimization

From: Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies

STD order

Factors coded

Response OD480

A

B

C

Actual value

Predicted value

1

−1

−1

0

3.492

3.562

2

1

−1

0

2.738

2.769

3

−1

1

0

2.274

2.244

4

1

1

0

2.536

2.466

5

−1

0

−1

2.419

2.393

6

1

0

−1

2.203

2.216

7

−1

0

1

2.574

2.561

8

1

0

1

2.139

2.165

9

0

−1

−1

3.319

3.275

10

0

1

−1

1.945

2.002

11

0

−1

1

2.928

2.871

12

0

1

1

2.479

2.523

13

0

0

0

2.013

2.075

14

0

0

0

2.273

2.075

15

0

0

0

1.996

2.075

16

0

0

0

1.892

2.075

17

0

0

0

2.202

2.075