Skip to main content
Bioresources and Bioprocessing

Table 6 Solubilization and refolding before and after optimization

From: Improving the mda-7/IL-24 refolding and purification process using optimized culture conditions based on the structure characteristics of inclusion bodies

Optimization items

Solubilization

Refolding

Absorbance at 480 nm

Increase of solubility (%)

Renatured mda-7/IL-24 (%)

Increase of refolding (%)

Control

3.921

-

13

-

Temperature

3.383

14

16

22

IPTG

3.672

6

14

9.0

pH

Not significant

-

-

-

Metal ion additives

1.94

51

23

84

Back to article page