Skip to main content

Table 2 Evaluation of physiological parameters for xylanase production from A. tubingensis FDHN1 under SSF

From: Optimization of upstream and downstream process parameters for cellulase-poor-thermo-solvent-stable xylanase production and extraction by Aspergillus tubingensis FDHN1

Variable

Substrate/moisture ratio a ( w / v )

 

1:1

1:2

1:3

1:4

1:5

1:6

1:7

 Xylanase activity (U/g)

1,064 ± 12

1,257 ± 8

1,356 ± 9

1,430 ± 13

1,659 ± 17

1,463 ± 6

1,397 ± 6

 Protein (mg/g)

16.34 ± 0.63

18.20 ± 0.46

16.69 ± 0.79

20.04 ± 0.87

20.33 ± 0.29

19.53 ± 0.67

18.29 ± 0.75

 Specific activity (U/mg)

65.11 ± 2.47

69.06 ± 3.21

81.24 ± 3.66

71.35 ± 2.59

81.60 ± 1.18

74.91 ± 3.34

76.38 ± 3.09

 Cellulase activity (U/g)

0.21 ± 0.04

0.53 ± 0.02

0.64 ± 0.01

1.53 ± 0.06

2.03 ± 0.11

2.59 ± 0.10

2.07 ± 0.15

Variable

pH b

 

3

4

5

6

7

8

9

 Xylanase activity (U/g)

978 ± 5

1,331 ± 12

1,606 ± 7

2,012 ± 18

1,924 ± 15

1,745 ± 13

1,565 ± 19

 Protein (mg/g)

16.87 ± 0.57

21.97 ± 0.92

25.01 ± 1.03

24.44 ± 0.87

26.27 ± 1.00

26.24 ± 0.81

24.03 ± 0.78

 Specific activity (U/mg)

57.97 ± 2.88

60.58 ± 1.24

64.21 ± 2.55

82.32 ± 2.31

73.25 ± 3.09

66.50 ± 2.99

65.12 ± 3.18

 Cellulase activity (U/g)

ND

ND

0.78 ± 0.08

1.64 ± 0.12

2.33 ± 0.07

3.17 ± 0.13

2.56 ± 0.13

Variable

Temperature c (°C)

 

25

30

35

40

45

50

55

 Xylanase activity (U/g)

1,452 ± 19

1,808 ± 15

1,860 ± 15

1,998 ± 12

1,623 ± 10

1,331 ± 15

1,056 ± 10

 Protein (mg/g)

25.29 ± 0.98

26.66 ± 1.21

27.93 ± 0.77

28.00 ± 0.61

24.33 ± 1.03

24.34 ± 1.14

23.33 ± 0.62

 Specific activity (U/mg)

57.41 ± 2.43

67.81 ± 2.08

66.59 ± 1.26

71.35 ± 2.97

66.70 ± 3.04

54.68 ± 1.33

45.26 ± 1.97

 Cellulase activity (U/g)

ND

0.64 ± 0.10

2.15 ± 0.08

2.87 ± 0.16

2.06 ± 0.13

1.31 ± 0.04

0.80 ± 0.06

  1. aSSF was carried out using 9 g of sorghum straw moistened with mineral salt medium (pH 5.0) at 35°C for 5 days; bSSF was carried out using 9 g of sorghum straw moistened with mineral salt medium of various pH values at 1:5 (w/v) moisture ratio at 35°C for 5 days; cSSF was carried out using 9 g of sorghum straw moistened with mineral salt medium (pH 6.0) for 5 days at various temperatures. ND, not determined. The data presented are the mean values of three replicates with the standard deviations.