Variable | Substrate/moisture ratio a ( w / v ) | ||||||
 | 1:1 | 1:2 | 1:3 | 1:4 | 1:5 | 1:6 | 1:7 |
 Xylanase activity (U/g) | 1,064 ± 12 | 1,257 ± 8 | 1,356 ± 9 | 1,430 ± 13 | 1,659 ± 17 | 1,463 ± 6 | 1,397 ± 6 |
 Protein (mg/g) | 16.34 ± 0.63 | 18.20 ± 0.46 | 16.69 ± 0.79 | 20.04 ± 0.87 | 20.33 ± 0.29 | 19.53 ± 0.67 | 18.29 ± 0.75 |
 Specific activity (U/mg) | 65.11 ± 2.47 | 69.06 ± 3.21 | 81.24 ± 3.66 | 71.35 ± 2.59 | 81.60 ± 1.18 | 74.91 ± 3.34 | 76.38 ± 3.09 |
 Cellulase activity (U/g) | 0.21 ± 0.04 | 0.53 ± 0.02 | 0.64 ± 0.01 | 1.53 ± 0.06 | 2.03 ± 0.11 | 2.59 ± 0.10 | 2.07 ± 0.15 |
Variable | pH b | ||||||
 | 3 | 4 | 5 | 6 | 7 | 8 | 9 |
 Xylanase activity (U/g) | 978 ± 5 | 1,331 ± 12 | 1,606 ± 7 | 2,012 ± 18 | 1,924 ± 15 | 1,745 ± 13 | 1,565 ± 19 |
 Protein (mg/g) | 16.87 ± 0.57 | 21.97 ± 0.92 | 25.01 ± 1.03 | 24.44 ± 0.87 | 26.27 ± 1.00 | 26.24 ± 0.81 | 24.03 ± 0.78 |
 Specific activity (U/mg) | 57.97 ± 2.88 | 60.58 ± 1.24 | 64.21 ± 2.55 | 82.32 ± 2.31 | 73.25 ± 3.09 | 66.50 ± 2.99 | 65.12 ± 3.18 |
 Cellulase activity (U/g) | ND | ND | 0.78 ± 0.08 | 1.64 ± 0.12 | 2.33 ± 0.07 | 3.17 ± 0.13 | 2.56 ± 0.13 |
Variable | Temperature c (°C) | ||||||
 | 25 | 30 | 35 | 40 | 45 | 50 | 55 |
 Xylanase activity (U/g) | 1,452 ± 19 | 1,808 ± 15 | 1,860 ± 15 | 1,998 ± 12 | 1,623 ± 10 | 1,331 ± 15 | 1,056 ± 10 |
 Protein (mg/g) | 25.29 ± 0.98 | 26.66 ± 1.21 | 27.93 ± 0.77 | 28.00 ± 0.61 | 24.33 ± 1.03 | 24.34 ± 1.14 | 23.33 ± 0.62 |
 Specific activity (U/mg) | 57.41 ± 2.43 | 67.81 ± 2.08 | 66.59 ± 1.26 | 71.35 ± 2.97 | 66.70 ± 3.04 | 54.68 ± 1.33 | 45.26 ± 1.97 |
 Cellulase activity (U/g) | ND | 0.64 ± 0.10 | 2.15 ± 0.08 | 2.87 ± 0.16 | 2.06 ± 0.13 | 1.31 ± 0.04 | 0.80 ± 0.06 |