Table 2 Primer sequence and characteristics of the seven loci used in MLST
Gene | primer | Sequence (5 ′- 3′) | Amplicon size (bp) | Amplicon bps (from - to) in NC_006958.1 | Allele size (bp) | Number of distinct alleles | Number of polymorphic sites |
|---|---|---|---|---|---|---|---|
atpA | atpA_F | ATGTACCAGGGCAACCAC | 390 | 1277256-1277645 | 240 | 6 | 13 |
atpA_R | GGCAACCTTCTTCATACC | ||||||
dnaE | dnaE_F | GGCGAAGGATATTCCGTTG | 366 | 2220485-2220120 | 225 | 6 | 23 |
dnaE_R | ACTTCCCCATCGCGGTTG | ||||||
dnaK | dnaK_F | CACCTCACAGGAAATCTC | 397 | 2959446-2959050 | 243 | 7 | 17 |
dnaK_R | GGACTGGAACTTCTCTAC | ||||||
fusA | fusA_F | CAAGGCAGCTATCCGTAAGATG | 402 | 524614-525015 | 237 | 7 | 11 |
fusA_R | ATGTTACCAGCGTGTGCAACC | ||||||
leuA | leuA_F | CTGGTTCAGGCTCGTGAGCA | 318 | 267591-267274 | 150 | 7 | 26 |
leuA_R | GATCATTGGGTTCTCAGGA | ||||||
odhA | odhA_F | CTTTGGCTGGGTCATGGAAGG | 327 | 1175355-1175681 | 186 | 5 | 5 |
odhA_R | CTGGGCATCGTGCCAGAAAC | ||||||
rpoB | rpoB_F | TATGTGACCGCGGAGTTC | 357 | 513278-513634 | 165 | 4 | 5 |
rpoB_R | GAAACGCTCGGTGATCTG |