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Table 1 PCR amplification protocol

From: Biosurfactant, polythene, plastic, and diesel biodegradation activity of endophytic Nocardiopsis sp. mrinalini9 isolated from Hibiscus rosasinensis leaves

94°C 94°C 55°C 72°C 72°C
5 min 30 s 30 s 1 min 5 min
Initial denaturation Denaturation Annealing Extension Final Extension
  35 cycles