Cellulose-degrading enzymes from Aspergillus terreus D34 and enzymatic saccharification of mild-alkali and dilute-acid pretreated lignocellulosic biomass residues

Kumar, Adepu K; Parikh, Bhumika S

doi:10.1186/s40643-015-0038-8

Bioresources and Bioprocessing

Table 4 Enzymatic saccharification of mild alkali and dilute acid pretreated lignocellulosic biomass residues

From: Cellulose-degrading enzymes from Aspergillus terreus D34 and enzymatic saccharification of mild-alkali and dilute-acid pretreated lignocellulosic biomass residues

Enzyme	Pretreatment condition		Rice straw ( RS )		Sugarcane bagasse ( BG )
Enzyme	Pretreatment condition		Reducing sugars ( g g ⁻¹ )	Saccharification (%)	Reducing sugars ( g g ⁻¹ )	Saccharification (%)
RS-grown cells	Alkali	24 h	0.621 ± 0.013^c	70.2 ± 1.5	0.249 ± 0.016^e	29.6 ± 1.9
	Alkali	48 h	0.733 ± 0.009^g	82.8 ± 1.0	0.312 ± 0.018^j	37.1 ± 2.1
	Acid	24 h	0.356 ± 0.015^k	40.9 ± 1.7	0.179 ± 0.011^k	30.8 ± 1.9
	Acid	48 h	0.424 ± 0.012^p	48.8 ± 1.4	0.216 ± 0.015^p	37.2 ± 2.6
BG-grown cells	Alkali	24 h	0.648 ± 0.008^b	73.2 ± 0.9	0.336 ± 0.021^d	40.0 ± 2.5
	Alkali	48 h	0.702 ± 0.011^h	79.3 ± 1.2	0.440 ± 0.016ⁱ	52.4 ± 1.9
	Acid	24 h	0.337 ± 0.021^l	38.8 ± 2.4	0.059 ± 0.005^m	10.3 ± 0.9
	Acid	48 h	0.352 ± 0.013^q	40.5 ± 1.5	0.112 ± 0.009^q	19.3 ± 1.5
Mixed growth cells (RS50:BG 50)	Alkali	24 h	0.454 ± 0.019^d	51.3 ± 2.1	0.368 ± 0.018^c	43.8 ± 2.1
	Alkali	48 h	0.646 ± 0.018ⁱ	73.0 ± 2.0	0.498 ± 0.014^h	59.3 ± 1.7
	Acid	24 h	0.293 ± 0.012^m	33.7 ± 1.4	0.121 ± 0.011^l	20.8 ± 1.9
	Acid	48 h	0.451 ± 0.026°	51.9 ± 3.0	0.201 ± 0.019^p	34.6 ± 3.3
Celluclast 1.5 L	Alkali	24 h	0.635 ± 0.008^b	71.8 ± 0.9	0.462 ± 0.006^b	55.0 ± 0.7
		48 h	0.826 ± 0.006^f	93.3 ± 0.7	0.690 ± 0.003^g	82.1 ± 0.4
	Acid	24 h	0.285 ± 0.010^m	32.8 ± 1.2	0.105 ± 0.004^l	18.1 ± 0.7
		48 h	0.460 ± 0.004°	52.9 ± 0.5	0.285 ± 0.010°	49.0 ± 1.7
Celluclast 1.5 L + Novozyme 188	Alkali	24 h	0.714 ± 0.008^a	80.7 ± 0.9	0.513 ± 0.008^a	61.0 ± 1.0
		48 h	0.854 ± 0.003^e	96.5 ± 0.3	0.742 ± 0.003^f	88.3 ± 0.4
	Acid	24 h	0.420 ± 0.012^j	48.3 ± 1.4	0.168 ± 0.011^k	28.9 ± 1.9
		48 h	0.648 ± 0.008ⁿ	74.5 ± 0.9	0.315 ± 0.006ⁿ	54.2 ± 1.0

At 10% solid loading and enzyme loadings^# of 9 FPU/g substrate using crude cellulase enzymes and formulated enzyme cocktails.
^#Enzyme loadings of fixed concentration (9FPU/g substrate) for respective biomass were obtained directly from the crude enzyme from the respective biomass sources. In each case, the final enzyme loadings were calculated based on the enzyme activities shown in Table 1. Commercial enzymes (Celluclast 1.5 L and Novozyme 188) were also tested for comparison. Each value represents the mean ± standard error; values with no common letter in a column (calculated separately for respective time, biomass, and pretreatment) are significantly different at p < 0.05.

Back to article page