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Table 4 Actual and coded level of variables tested with Box-Behnken design for α-L-arabinofuranosidase production

From: Improved yield of α-L-arabinofuranosidase by newly isolated Aspergillus niger ADH-11 and synergistic effect of crude enzyme on saccharification of maize stover

Process variable

Coded level of variable

−1

0

+1

Urea (X1)

0.1

2.55

5

MgSO4 (X2)

0.1

1.55

3

CaCl2 (X3)

0.1

1.55

3

pH (X4)

3

5.5

8

Ammonium sulfate (X5)

1

5.5

10

Fermentation time (X6)

48

132

216