Table 5 Full factorial BBD matrix for α-L-arabinofuranosidase production by Aspergillus niger ADH-11
Urea | MgSO 4 | CaCl 2 | pH | Ammonium sulfate | Fermentation time | α-L-Arabinofuranosidase | ||
|---|---|---|---|---|---|---|---|---|
Run | X1 | X2 | X3 | X4 | X5 | X6 | Predicted activity (U/g) | Experimental activity (U/g) |
1 | −1 | −1 | 0 | −1 | 0 | 0 | 24.803 | 23.750 |
2 | 1 | −1 | 0 | −1 | 0 | 0 | 23.506 | 21.940 |
3 | −1 | 1 | 0 | −1 | 0 | 0 | 21.359 | 24.070 |
4 | 1 | 1 | 0 | −1 | 0 | 0 | 22.517 | 20.990 |
5 | −1 | −1 | 0 | 1 | 0 | 0 | 21.465 | 22.510 |
6 | 1 | −1 | 0 | 1 | 0 | 0 | 22.089 | 19.860 |
7 | −1 | 1 | 0 | 1 | 0 | 0 | 20.756 | 21.840 |
8 | 1 | 1 | 0 | 1 | 0 | 0 | 23.835 | 25.370 |
9 | 0 | −1 | −1 | 0 | −1 | 0 | 18.686 | 20.850 |
10 | 0 | 1 | −1 | 0 | −1 | 0 | 18.814 | 20.140 |
11 | 0 | −1 | 1 | 0 | −1 | 0 | 19.025 | 19.720 |
12 | 0 | 1 | 1 | 0 | −1 | 0 | 14.183 | 12.240 |
13 | 0 | −1 | −1 | 0 | 1 | 0 | 16.684 | 17.410 |
14 | 0 | 1 | −1 | 0 | 1 | 0 | 19.828 | 20.350 |
15 | 0 | −1 | 1 | 0 | 1 | 0 | 21.293 | 18.750 |
16 | 0 | 1 | 1 | 0 | 1 | 0 | 19.467 | 18.520 |
17 | 0 | 0 | −1 | −1 | 0 | −1 | 20.493 | 17.850 |
18 | 0 | 0 | 1 | −1 | 0 | −1 | 17.673 | 19.390 |
19 | 0 | 0 | −1 | 1 | 0 | −1 | 12.448 | 13.680 |
20 | 0 | 0 | 1 | 1 | 0 | −1 | 16.328 | 15.430 |
21 | 0 | 0 | −1 | −1 | 0 | 1 | 22.543 | 23.930 |
22 | 0 | 0 | 1 | −1 | 0 | 1 | 18.641 | 16.920 |
23 | 0 | 0 | −1 | 1 | 0 | 1 | 21.868 | 20.640 |
24 | 0 | 0 | 1 | 1 | 0 | 1 | 24.666 | 26.820 |
25 | −1 | 0 | 0 | −1 | −1 | 0 | 16.175 | 16.160 |
26 | 1 | 0 | 0 | −1 | −1 | 0 | 17.414 | 17.340 |
27 | −1 | 0 | 0 | 1 | −1 | 0 | 14.703 | 12.370 |
28 | 1 | 0 | 0 | 1 | −1 | 0 | 17.861 | 17.410 |
29 | −1 | 0 | 0 | −1 | 1 | 0 | 19.621 | 19.590 |
30 | 1 | 0 | 0 | −1 | 1 | 0 | 18.245 | 21.060 |
31 | −1 | 0 | 0 | 1 | 1 | 0 | 17.154 | 17.710 |
32 | 1 | 0 | 0 | 1 | 1 | 0 | 17.697 | 17.230 |
33 | 0 | −1 | 0 | 0 | −1 | −1 | 15.256 | 15.060 |
34 | 0 | 1 | 0 | 0 | −1 | −1 | 15.484 | 14.150 |
35 | 0 | −1 | 0 | 0 | 1 | −1 | 15.089 | 14.820 |
36 | 0 | 1 | 0 | 0 | 1 | −1 | 18.333 | 16.440 |
37 | 0 | −1 | 0 | 0 | −1 | 1 | 22.735 | 23.410 |
38 | 0 | 1 | 0 | 0 | −1 | 1 | 17.793 | 19.280 |
39 | 0 | −1 | 0 | 0 | 1 | 1 | 23.168 | 25.720 |
40 | 0 | 1 | 0 | 0 | 1 | 1 | 21.242 | 20.220 |
41 | −1 | 0 | −1 | 0 | 0 | −1 | 16.569 | 15.160 |
42 | 1 | 0 | −1 | 0 | 0 | −1 | 16.174 | 18.470 |
43 | −1 | 0 | 1 | 0 | 0 | −1 | 13.391 | 15.960 |
44 | 1 | 0 | 1 | 0 | 0 | −1 | 20.412 | 21.240 |
45 | −1 | 0 | −1 | 0 | 0 | 1 | 24.726 | 23.410 |
46 | 1 | 0 | −1 | 0 | 0 | 1 | 19.487 | 16.430 |
47 | −1 | 0 | 1 | 0 | 0 | 1 | 20.467 | 18.660 |
48 | 1 | 0 | 1 | 0 | 0 | 1 | 22.643 | 24.540 |
49 | 0 | 0 | 0 | 0 | 0 | 0 | 18.998 | 21.490 |
50 | 0 | 0 | 0 | 0 | 0 | 0 | 18.998 | 16.630 |
51 | 0 | 0 | 0 | 0 | 0 | 0 | 18.998 | 20.180 |
52 | 0 | 0 | 0 | 0 | 0 | 0 | 18.998 | 17.460 |
53 | 0 | 0 | 0 | 0 | 0 | 0 | 18.998 | 18.670 |
54 | 0 | 0 | 0 | 0 | 0 | 0 | 18.998 | 19.560 |