An overview on biofuel and biochemical production by photosynthetic microorganisms with understanding of the metabolism and by metabolic engineering together with efficient cultivation and downstream processing

Sarkar, Dayanidhi; Shimizu, Kazuyuki

doi:10.1186/s40643-015-0045-9

Bioresources and Bioprocessing

Table 1 Biofuel and biochemical production by cyanobacteria

From: An overview on biofuel and biochemical production by photosynthetic microorganisms with understanding of the metabolism and by metabolic engineering together with efficient cultivation and downstream processing

Product	Species	Titer or productivity	Overexpressed or knockout gene (s)	Cultivation	Reference
Ethanol	Synechococcus	230 mg/L in 28 days	pdc, adh	Shake flask	[210]
	Synechocystis	552 mg/L in 6 days	pdc, adh	Photobioreactor	[127]
	Synechocystis	608 mg/L in 18 days	pdc, adh	Photobioreactor	[128]
Isobutyraldehyde	Synechococcus	1,100 mg/L in 8 days	alsS, ilvC,D, kivd,rbcls	Bottle with NaHCO3	[61]
Isobutanol	Synechococcus	18 mg/L	kivd, yqhD	Shake flask with NaHCO3	[61]
	synechococcus	450 mg/L in 6 days	alsS, ilvC, D, kivd, yqhD	Shake flask with NaHCO3	[61]
2 Methyl-1-butanol	Synechococcus	2 mg/L	kivd, yqhD, cims	Shake flask with NaHCO3	[61,130]
1-Butanol	Synechococcus	14.5 mg/L in 7 days	hbd, crt, adhE2, ter, atoB	Bottle under anoxic cond.	[131]
	Synechococcus	30 mg/L in 18 days	ter, nphT7, bldh, yqhD, phaJ, B	Shake flask	[132]
Fatty alcohol	Synechocystis	0.2 mg/L in 18 days	far	Photobaioreactor with 5% CO₂	[143]
	Synechocystis	0.02 mg/L/OD	far,aas	Shake flask	[140]
	Synechocystis	2.87 mg/gDCW	Δsll0208, Δsll0209	Flask	[211]
Fatty acids	Synechocystis	197 mg/L in 17 days	tesA, accBCDA, fatB1, B2, tesA137	1% CO₂ bubbling	[142]
Alka (e) nes	Synechocystis	0.162 mg/L/OD	accBCDA	Shake flask	[143]
	Synechocystis	2.3 mg/l/OD	sll0208, sll0209	Shake flask	[212]
Hydrogen	Synechococcus	2.8 μmol/h/mgChlorophyll-a	hydEF, hydG, hydA	Anaerobic condition	[153]
	Synechococcus	54 mmol/1,017 cells in 4 days	Δldh	Anoxic condition	[213]
L-Lactate	Synechocystis	0.0178 mmol/gDCW/h	ldh, sth	Shaking incubator	[214]
	Synechocystis	0.2512 mmol/gDCW/h	pyk, ldh	Shaking incubator	[136]
D-Lactate	Synechocystis	2.17 g/L in 24 days	gldA, sth	Photoautotropic with acetate	[215]
1,2-propanediol	Synechococcus	Approximately 150 mg/L	mgsA, gldA, yqhD	Shake flask	[216]
Isoprene	Synechocystis	50 μg/gDCW/d	lspS	Sealed culture	[138]
Ethylene	Synechocystis	26 μmol/gDCW/h	efe (RS1010)	Rotary shaker	[217]
	Synechocystis	111.6 μmol/gDCW/h	efe (slr068)	Rotary shaker	[217]
	Synechococcus	84.8 μmol/gDCW/h	efe (pUC303)	Flask	[218]
	Synechococcus	80.5 μmol/gDCW/h	efe (psbAl)	Flask	[219]
Acetone	Synechocystis	36.0 mg/L in 4 days	ctfAB, adc, ΔphaCE, Δpta	Flask	[148]
PHAs	Synechocystis	1.4 mg/100 mgDCW	sigE	Bubbled with 1% CO₂ in the air	[106]
	Synechocystis	533.4 mg/L in 21 days	Δslr1829, Δslr1830	Flask	[220]

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