Figure 2
From: Assembly of cellulases with synthetic protein scaffolds in vitro
Purification of interaction proteins and their binding affinity. (A) SDS-PAGE analysis of purified interaction proteins. Biolayer interferometry sensorgrams of (B) IPA-IPa, (C) IPB-IPb, (D) IPC-IPc. IPA, IPB, and IPC were immobilized to the streptavidin-coated biosensors, respectively. Then IPa, IPb, and IPc were bound to the corresponding proteins, respectively. The figure shows the response of association and dissociation. All the response value was normalized by minusing the value of control.