Table 1 Primers used in this work
From: Assembly of cellulases with synthetic protein scaffolds in vitro
Name | Sequence |
|---|---|
IPA-F | GGAATTCCATATGATGCGAATTCTGGCTATC |
IPA-R | CCGCTCGAGTTCTTTGCCCGGAAGTTTC |
IPa-F | GGAATTCCATATGATGCGTGTACTGGGTATTG |
IPa-R | CCGCTCGAGCGCAGCCGGTAACTCCGCC |
IPB-F | GGAATTCCATATGATGAACGATGAAATGAAAG |
IPB-R | CCGCTCGAGGGCTTTATTCTGACGCCAC |
IPb-F | GGAATTCCATATGATGGCAAGATATTTGGG |
IPb-R | CCGCTCGAGCTTGGAGTAAAGCTCGAC |
IPC-F | GGAATTCCATATGATGACTGACAAAATGC |
IPC-R | CCGCTCGAGCGCTTCAATGGCAGCAC |
IPc-F | GGAATTCCATATGATGGCAACACGAATTG |
IPc-R | CCGCTCGAGTGGAATCAGCAGGCTGG |
A-F | GGAATTCCATATGTATGATGCTTCACTTATTCCG |
A-R | CCGCTCGAGGTTGCTTGGAAGCTTAC |
E-F | GTGCCGCGCGGCAGCCATATGATAGGACAAGCATTTGC |
E-R | GTGGTGGTGGTGGTGCTCGAGAGTTGGAGGAGTCACTGACCC |
4-F | GGAATTCCATATGCAATACGATCAGATAGATAAA |
4-R | CCGCTCGAGCAGAGCAAGAGCTATAG |
IPaA-F | GTGCCGCGCGGCAGCATGCGTGTACTGGGTATTGAAACTTC |
IPaA-R | AAGTGAAGCATCATACGATCCGCCACCGCCAGAGCCACCTCCGCCCGCAGCCGGTAACTCCG |
AIPa-F | AAGCTTCCAAGCAACGGCGGAGGTGGCTCTGGCGGTGGCGGATCGCGTGTACTGGGTATTG |
AIPa-R | GTGGTGGTGGTGGTGCGCAGCCGGTAACTCCGCCAGCGGC |
IPbE-F | GTGCCGCGCGGCAGCATGGCAAGATATTTGGGTCCTAAGC |
IPbE-R | AAATGCTTGTCCTATCGATCCGCCACCGCCAGAGCCACCTCCGCCCTTGGAGTAAAGCTCG |
EIPb-F | GTGACTCCTCCAACTGGCGGAGGTGGCTCTGGCGGTGGCGGCAAGATATTTGGGTCCTAAGC |
EIPb-R | GTGGTGGTGGTGGTGCTTGGAGTAAAGCTCGACGATCAGGTG |
IPc4-F | GTGCCGCGCGGCAGCATGACTGACAAAATGCAAAGTTTAG |
IPc4-R | TATCTGATCGTATTGCGATCCGCCACCGCCAGAGCCACCTCCGCCCGCTTCAATGGCAGCAC |
4IPc-F | ATAGCTCTTGCTCTGGGCGGAGGTGGCTCTGGCGGTGGCGGATCGACTGACAAAATGCAAAG |
4IPc-R | GTGGTGGTGGTGGTGCGCTTCAATGGCAGCACGCAATTTTTTC |
ScafBAC-F1 | TAAGAAGGAGATATAAACGATGAAATGAAAGGTAAAAGCGGC |
ScafBAC-F2 | CGTCAGAATAAAGCCGGCGGAGGTGGCTCTGGCGGTGGCGGATCGCGAATTCTGGCTATCG |
ScafBAC-F3 | CTTCCGGGCAAAGAAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGACTGACAAAATGCAAAGTT |
ScafBAC-R | GTGGTGGTGGTGGTGCTCGAGCGCTTCAATGGCAGCACGC |
ScafABC-F1 | TAAGAAGGAGATATACGAATTCTGGCTATCGATACCGCGACAGAGGCCTGCTC |
ScafABC-F2 | CTTCCGGGCAAAGAAGGCGGAGGTGGCTCTGGCGGTGGCGGATCGAACGATGAAATGAAAGG |
ScafABC-F3 | CGTCAGAATAAAGCCGGCGGAGGTGGCTCTGGCGGTGGCGGATCGACTGACAAAATGCAAAG |
ScafBCA-F | GCTGCCATTGAAGCGGGCGGAGGTGGCTCTGGCGGTGGCGGATCGCGAATTCTGGCTATCG |
ScafBCA-R | GTGGTGGTGGTGGTGTTCTTTGCCCGGAAGTTTCTTCCATGC |