Skip to main content
Figure 4 | Bioresources and Bioprocessing

Figure 4

From: An immunochromatographic test strip for rapid detection of fish pathogen Edwardsiella tarda

Figure 4

Dot blotting detection with monoclonal antibody 25C1 against E. tarda. Bacterial strains used for the cross-reactivity test at a concentration of 1 × 106 CFU/ml and E. tarda EIB202 of serial dilutions were spotted onto the nitrocellulose membranes (2 μl/spot) and processed dot blotting using MAb 25C1. Row 1: E. tarda EIB202 (a) 1 × 106 CFU/ml, (b) 5 × 105 CFU/ml, (c) 1 × 105 CFU/ml, (d) 5 × 104 CFU/ml, (e) 1 × 104 CFU/ml, (f) 5 × 103 CFU/ml, (g) 1 × 103 CFU/ml. Row 2: (a) E. tarda EIB202, (b) E. tarda WY37, (c) E. tarda ATCC15947, (d) E. tarda ATCC23692, (e) E. tarda DT, (f) E. ictaluri LH-51, (g) E. ictaluri 33202. Row 3: (a) V. anguillarum MVM425, (b) V. anguillarum VIB72, (c) V. anguillarum AV-1, (d) E. coli CC118, (e) E. coli DH5α, (f) C. violaceum CV026, (g) A. hydrophila. Row 4: (a) V. parahaemolyticus, (b) V. alginolyticus EPGS, (c) V. harveyi VIB647, (d) blank.

Back to article page