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Table 1 Delignification methods using ligninolytic fungi and ligninolytic enzymes

From: Ligninolytic enzymes: a biotechnological alternative for bioethanol production

Feedstock Fungal strain Type of process Mediator Delignification % Glucose yield Ethanol yield Process duration Ref.
Wood pulp Trametes villosa Enzymatic Laccase HBT
HPA
50%
39%
- - 15 h [81]
Wood pulp Aspergillus fumigatus Enzymatic Laccase HBT 14% - - 2 h [59]
Hardwood chips Trametes versicolor Enzymatic Laccase VA 54.2%, - - 2 h [82]
Sugarcane bagasse Ceriporiopsis subvermispora Fungal
MnP,
LiP,
Laccase
- 20% - - 30 d [64]
Eucalyptus globulus Myceliophthora thermophila Enzymatic Laccase MS >25% - - 12 h [19]
Paper pulp Pycnoporus cinnabarinus Enzymatic Laccase AC
SA
CA
HBT
21%
25%
−9%
40%
- - 4-24 h [69]
Corn stover
Switchgrass
Ceriporiopsis subvermispora Fungal Laccase - >25%
>25%
56%
37%
- 18 d [4]
Ricinus communis - Enzymatic Laccase - 85.69% 288.83
mg/g
- 4 h [83]
Eucalyptus globulus Pycnoporus cinnabarinus Enzymatic Laccase HBT 27% - - 2-4 h [84]
Wheat straw Isolate Euc,
Irpex lacteus
Fungal Laccase - 35%
35%
70%
70%
- 15-46 d [2]
Wheat straw Ceriporiopsis subvermispora
Irpex lacteus
Fungal
MnP,
LiP,
Laccase
- 34%
39%
69%
66%
90% 21 d [3]
Corn stover Ceriporiopsis subvermispora1 Fungal
MnP
Laccase
- 39.2% 66.9% - 42 d [7]
Corn stover (cs)
Wheat straw (ws)
Pycnoporus sanguineus H275 Fungal
Laccase
-
-
VA
27% (cs)
25% (ws)
71% (cs)
72% (ws)
- - 21 d [8]
Elephant grass (eg)
Eucalypt wood (ew)
Trametes villosa LMS HBT 36% (eg)
58% (ew)
70.7% (eg)
57.8 (ew)
16.2% (eg)
12.3% (ew)
24 h [72]
Corn stover Trametes versicolor LMS HBT - 31.7% - 48 h [70]
Wheat straw Sclerotium sp. LMS ABTS - 82.29% - 48 h [71]
Cotton gin trash Trametes versicolor LMS PrimaGreen® 15% 23% 31.6% 96 h [27]
Cotton gin trash Trametes versicolor LMS PrimaGreen® 27% 41% 64% 96 h [28]
Sugarcane bagasse Pleurotus ostreatus IBL-02 Enzymatic MnP,
LiP,
Laccase
- 33.6% 72.9% 16.3 g/L 48 h [67]
Wheat straw T. versicolor
I. lacteus
Fungal
MnP
LiP
Laccase
- - 68.33%
56.26%
- 21 d [85]
Sugarcane bagasse Phlebia sp MG-60 IFF   30% - 45% 4 weeks [86]
Sugarcane bagasse Phanerochaete chrysosporium
Lentinula edode
Pleurotus ostreatus
Fungal
MnP
- 64% - - 12 weeks [87]
Oil palm empty fruit bunches Pleurotus floridanus Fungal   5% 15.4% 27.9% 4 weeks [88]
Corn stover Coprinus comatus IFF   66.5% 82%   5-7 d [76]
Wheat straw Irpex lacteus Fungal
MnP
Laccase
- - 84% 74% 14-21 d [62]
Corn stover Phanerochete chrysosporium Coridus versicolor Enzymatic MnP
Laccase
- - 323 mg/g - 24 h [66]
Bamboo culms Punctularia sp. Fungal   - 10% - 12 weeks [89]
Poplar wood Trametes velutina Fungal -   43.6% 22.2% 8 weeks [90]
Sugarcane bagasse Pleurotus florida Fungal
MnP
Laccase
- 7.91% 303.33 mg/g - 25 d [91]
Corn stover Irpex lacteu Fungal
MnP
  25.48% 308.98 mg/g 144.03 mg/g 28 d [92]
Sugarcane
Bagasse
Phlebia sp. MG-60 IFF - - - 44% 4 weeks [75]
Hardwood kraft pulp,
Waste newspaper
Phlebia sp. MG-60 IFF - - - 71.8%
51.1%
68 h [73]
Sugarcane bagasse Phlebia sp. MG-60 IFF - - - 65.7% 20 d [61]
Oak wood Phlebia sp. MG-60 IFF - - - 43.9% 56 d [74]
  1. LMS: Laccase-Mediator System, IFF: Integrated fungal fermentation, HBT: 1-Hydroxybenzotriazole, HPA: N-Hydroxyphthalimide, MS: Methyl syringate, VA: Violuric acid, AC: Acetosyringone, SA: Syringaldehyde, CA: p-coumaric acid.