Table 2 Primers used in this study
From: Secretive expression of heterologous β-glucosidase in Zymomonas mobilis
Target fragments | Primers | Sequences (5′–3′) |
|---|---|---|
bglB | bgl1 | CGGGATCCATGCGCAACTTGACCAAGAC (BamHІ) |
bgl2 | CCCAAGCTTTTAAAACCCGTTCTTCGCC (HindШ) | |
Peno | pe1 | CGGAATTCTCGGCCATTGTCTATACTC (EcoRI) |
pe2 | CGGGATCCATCGAAACCTTTCTTAAAATC (BamHI) | |
Peno-NprB | pn1 | CGGAATTCTCGGCCATTGTCTATACTC (EcoRI) |
pn2 | CGGGATCCTGCAGCTGAGGCATGTGTT (BamHI) | |
ZMO0186 | zc1 | CGGGATCCATGACCTATAGTCGTCGTTTTATC (BamHI) |
zc2 | CCCAAGCTTAAAGATTTAGCGGTTATGCG (HindШ) | |
Peno-SP0186 | pes1 | CGGAATTCTCGGCCATTGTCTATACTCCAGTTAC (EcoRI) |
pes2 | CGGGATCCGGCCGCTTTACCCTTCCG (BamHI) | |
sacB-bglB | p1 | CGGGATCCATGTTGAATAAAGCAGGCATTG (BamHI) |
p2 | ATAAAGGTATTCTCGCTCATACCACCACCACCACCACCACCTTTATTCAATAAAGACAGGGCT | |
p3 | TGTCTTTATTGAATAAATAAGGTGGTGGTGGTGGTGGTGGTATGAGCGAGAATACCTTTATATTTC | |
p4 | CCAAGCTTTTAAAACCCGTTCTTCGCC (HindШ) |