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Table 2 Primers used in this study

From: Secretive expression of heterologous β-glucosidase in Zymomonas mobilis

Target fragments Primers Sequences (5′–3′)
bglB bgl1 CGGGATCCATGCGCAACTTGACCAAGAC (BamHІ)
  bgl2 CCCAAGCTTTTAAAACCCGTTCTTCGCC (HindШ)
Peno pe1 CGGAATTCTCGGCCATTGTCTATACTC (EcoRI)
  pe2 CGGGATCCATCGAAACCTTTCTTAAAATC (BamHI)
Peno-NprB pn1 CGGAATTCTCGGCCATTGTCTATACTC (EcoRI)
  pn2 CGGGATCCTGCAGCTGAGGCATGTGTT (BamHI)
ZMO0186 zc1 CGGGATCCATGACCTATAGTCGTCGTTTTATC (BamHI)
  zc2 CCCAAGCTTAAAGATTTAGCGGTTATGCG (HindШ)
Peno-SP0186 pes1 CGGAATTCTCGGCCATTGTCTATACTCCAGTTAC (EcoRI)
  pes2 CGGGATCCGGCCGCTTTACCCTTCCG (BamHI)
sacB-bglB p1 CGGGATCCATGTTGAATAAAGCAGGCATTG (BamHI)
  p2 ATAAAGGTATTCTCGCTCATACCACCACCACCACCACCACCTTTATTCAATAAAGACAGGGCT
  p3 TGTCTTTATTGAATAAATAAGGTGGTGGTGGTGGTGGTGGTATGAGCGAGAATACCTTTATATTTC
  p4 CCAAGCTTTTAAAACCCGTTCTTCGCC (HindШ)
  1. The underlined sections mean the restriction enzyme sequences indicated by the parentheses