Table 1 Effect of phase-forming salts on glucoamylase partitioning
From: Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system
Salts | Phase volume ratio | Specific activity (U/mg) | Yield (%) | Purification factor | |
|---|---|---|---|---|---|
Top | Bottom | ||||
Trisodium citrate | 0.77 | 16.8 ± 0.73 | 21.06 ± 1.66 | 82.23 ± 1.10 | 1.12 ± 0.09 |
Tripotassium citrate | 1.07 | 12.92 ± 1.23 | 19.22 ± 1.02 | 75.75 ± 2.19 | 1.02 ± 0.01 |
Magnesium sulfate | 0.55 | 16.98 ± 1.72 | 15.88 ± 0.16 | 69.73 ± 1.77 | 0.85 ± 0.04 |
K2HPO4/KH2PO4 (1.82:1) | 0.72 | 10.82 ± 1.58 | 27.34 ± 0.51 | 82.48 ± 0.97 | 1.46 ± 0.09 |