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Table 1 Effect of phase-forming salts on glucoamylase partitioning

From: Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

Salts Phase volume ratio Specific activity (U/mg) Yield (%) Purification factor
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Trisodium citrate 0.77 16.8 ± 0.73 21.06 ± 1.66 82.23 ± 1.10 1.12 ± 0.09
Tripotassium citrate 1.07 12.92 ± 1.23 19.22 ± 1.02 75.75 ± 2.19 1.02 ± 0.01
Magnesium sulfate 0.55 16.98 ± 1.72 15.88 ± 0.16 69.73 ± 1.77 0.85 ± 0.04
K2HPO4/KH2PO4 (1.82:1) 0.72 10.82 ± 1.58 27.34 ± 0.51 82.48 ± 0.97 1.46 ± 0.09
  1. Phase system 15 % (w/w) PEG 4000 + 15 % (w/w) salt