Table 2 Effect of PEG molecular weight on glucoamylase partitioning
From: Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system
PEG molecular weight | Phase volume ratio | Specific activity (U/mg) | Yield (%) | Purification factor | |
|---|---|---|---|---|---|
Top | Bottom | ||||
PEG 1000 | 0.96 | 15.06 ± 1.33 | 19.16 ± 0.92 | 61.68 ± 0.94 | 1.14 ± 0.02 |
PEG 2000 | 0.78 | 13.21 ± 0.43 | 21.38 ± 1.23 | 73.31 ± 1.69 | 1.28 ± 0.11 |
PEG 4000 | 0.72 | 10.82 ± 1.58 | 27.34 ± 0.51 | 82.48 ± 0.97 | 1.46 ± 0.09 |
PEG 6000 | 0.65 | 9.57 ± 1.02 | 21.87 ± 0.83 | 84.01 ± 1.24 | 1.31 ± 0.05 |