Table 4 Comparisons of enzymology properties of polygalacturonase reported in the literatures
No. | Strains | Optimal temperature (°C) | Optimal pH | K m (mg/mL) | Stability | Refs. |
|---|---|---|---|---|---|---|
1 | Sckrotinia sclerotiorum | 45 | 5.0 | 0.83 | Mostly inactive at 65 °C, | [42] |
2 | Aspergillus fumigatus strain 4 | 65 | 3.5–4.5 | - | Stable between 3.0 and 9.0 and at 4–6 °C; activity reduce by 24 % at 26–30 °C | [39] |
3 | Aspergillus niger | 43 | 3.8–4.3 | 0.12 | T m = 43 °C | [18] |
4 | Aspergillus niger | 45 | 3, 4.6 | 0.72 | T m = 46 °C | [18] |
5 | Penicillium viridicatum Rfc3 | 55 | 5 | – | Stable in neutral pH range and at 40 ºC for 1 h | [43] |
6 | Thermoascus aurantiacus 179-5 | 65 | 5 | – | Stable in the acidic to neutral pH range at 60 °C for 1 h | [44] |
7 | Penicillium SPC-F 20 | 60 | 5.5 | – | A residual activity of 48 % after 2 h of incubation at 60 °C | [22] |
8 | Rhizopus microsporus var. rhizopodiformis | 65 | 3.5 | Activity decreased by 28 and 39 at 60 and 65 °C, respectively, for 90-min incubation | [45] | |
9 | Thermoascus aurantiacus | 60 | 5.0 | - | Stable in pH 3.0–4.5 at 55 °C for 1 h | [1] |
10 | Rhizomucor pusilis | 55 | 5.0 | 0.22 | Stable up to 50 °C for 120 min at pH 4.0–5.0; decreased rapidly above 60 °C and above pH 5.0 | [8] |
11 | Penicillium oxalicum SX6 | 50 | 5.0 | – | Stable at pH 3.5–6.0 and 40 °C | [13] |
12 | Aspergillus fumigatus | 60 | 5.0 | 35 | Stable at pH 2.0–7.0 and 60 °C for 2 h | In our work |