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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Use of combined UV and chemical mutagenesis treatment of Aspergillus terreus D34 for hyper-production of cellulose-degrading enzymes and enzymatic hydrolysis of mild-alkali pretreated rice straw

Fig. 1

SDS-PAGE analysis of RS- and BG-grown culture extracts. a RS-grown culture extract and b BG-grown culture extract. Irrespective of protein concentrations, each well was loaded with equal volume (20 µL) of sample. Lane 1 wild type; lane 2 UV1; lane 3 UV2; lane 4 EMS1; and lane 5 EMS2. c 10% separating gel. Lane 1 molecular markers; lane 2 wild-type BG; and lane 3 wild-type RS. Low-range molecular markers contain phosphorylase b (97.4 kDa), bovine serum albumin (66.6 kDa), ovalbumin (45 kDa), and carbonic anhydrase (31 kDa). d 4–20% gradient gel. Lane 1 and 4 molecular markers; lane 2 wild-type BG; and lane 3 wild-type RS. Broad range prestained molecular markers contains myoglobin (200 kDa), β-galactosidase (116 kDa), bovine serum albumin (66 kDa), ovalbumin (45 kDa), carbonic anhydrase (31 kDa), soybean trypsin inhibitor (21.5 kDa), lysozyme (14.4 kDa), and aprotinin (6.5 kDa). Molecular markers were purchased from Bio-Rad (Bio-Rad laboratories, USA).

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